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Использование первичных культивированных нейронов гиппокампа для изучения сборки начальных сегментов аксона

JoVE Journal
Neurociência

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JoVE Journal Neurociência

Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments

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Использование первичных культивированных нейронов гиппокампа для изучения сборки начальных сегментов аксона

4,790 Views

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06:53 min

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February 12, 2021

DOI:

10.3791/61411-v

DOI:

10.3791/61411-v

•

06:53 min

February 12, 2021

•

4 Views

Rui Yang^2,3, Vann Bennett³

¹School of Basic Medical Sciences,Xi’an Jiaotong University, ²Department of Cell Biology,Duke University, ³Department of Biochemistry,Duke University

Tags

Primary Cultured Hippocampal Neurons Axon Initial Segments Ankyrin-G CRE BFP DNA Ankyrin-G GFP DNA Transfection Dissection Trypsin Digestion Trituration Neuronal Culture Glial Cell Feeder Dishes

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