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Verwendung von primär kultivierten Hippocampus-Neuronen zur Untersuchung der Anordnung von Axon-Anfangssegmenten
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Neurociência
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JoVE Journal Neurociência
Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments
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Verwendung von primär kultivierten Hippocampus-Neuronen zur Untersuchung der Anordnung von Axon-Anfangssegmenten

4,792 Views

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06:53 min

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February 12, 2021

DOI:

10.3791/61411-v

DOI:
10.3791/61411-v

•

06:53 min
February 12, 2021

•

4 Views
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1School of Basic Medical Sciences,Xi’an Jiaotong University, 2Department of Cell Biology,Duke University, 3Department of Biochemistry,Duke University

Tags

Primary Cultured Hippocampal NeuronsAxon Initial SegmentsAnkyrin-GCRE BFP DNAAnkyrin-G GFP DNATransfectionDissectionTrypsin DigestionTriturationNeuronal CultureGlial Cell Feeder Dishes

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Cite this Article

Yang, R., Bennett, V. Use of Primary Cultured Hippocampal Neurons to Study the Assembly of Axon Initial Segments. J. Vis. Exp. (168), e61411, doi:10.3791/61411 (2021).

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