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Immunology and Infection

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JoVE Journal Immunology and Infection
Live-Cell Forward Genetische Benadering voor identificeren en isoleren developmental mutanten in Chlamydia trachomatis
 
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Live-Cell Forward Genetische Benadering voor identificeren en isoleren developmental mutanten in Chlamydia trachomatis

Article DOI: 10.3791/61365-v • 10:32 min • June 10th, 2020
June 10th, 2020 •
1, 1, 1
1Department of Biological Sciences, University of Idaho

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Dit protocol maakt gebruik van fluorescerende promotor-verslaggevers, live-cel microscopie, en individuele opname extractie in een gerichte voorwaartse genetische benadering te identificeren en isoleren van ontwikkelingsmutanten van Chlamydia trachomatis.

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Immunologie en infectie Probleem 160 Forward genetica chemische mutagenesis chlamydial ontwikkeling live-cel microscopie geautomatiseerde microscopie fluorescerende verslaggevers
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