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Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae
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Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae
Capítulos
- 00:04Introduction
- 00:32Preparation of Third-instar Larvae (L3)
- 02:24Thermogenetic Activation and No-choice Feeding Assay
- 03:35Food Dye Extraction and Colorimetric Quantification of Food Consumption
- 05:37Results: Amount of Food Eaten per Larva under Neuronal Thermogenetic-activation Conditions in Three Macronutrient Balancing Diets
- 06:49Conclusion
Described here is a protocol that enables the colorimetric quantification of the amount of food eaten within a defined interval of time by Drosophila melanogaster larvae exposed to diets of different macronutrient quality. These assays are conducted in the context of a neuronal thermogenetic screen.
