Described here is a protocol that enables the colorimetric quantification of the amount of food eaten within a defined interval of time by Drosophila melanogaster larvae exposed to diets of different macronutrient quality. These assays are conducted in the context of a neuronal thermogenetic screen.
Poças, G. M., Domingos, P. M., Mirth, C. K. Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae. J. Vis. Exp. (160), e61323, doi:10.3791/61323 (2020).