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Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae
JoVE Revista
Neurociencias
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JoVE Revista Neurociencias
Quantification of Macronutrients Intake in a Thermogenetic Neuronal Screen using Drosophila Larvae
DOI:

07:24 min

June 11, 2020

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Capítulos

  • 00:04Introduction
  • 00:32Preparation of Third-instar Larvae (L3)
  • 02:24Thermogenetic Activation and No-choice Feeding Assay
  • 03:35Food Dye Extraction and Colorimetric Quantification of Food Consumption
  • 05:37Results: Amount of Food Eaten per Larva under Neuronal Thermogenetic-activation Conditions in Three Macronutrient Balancing Diets
  • 06:49Conclusion

Summary

Traducción Automática

Described here is a protocol that enables the colorimetric quantification of the amount of food eaten within a defined interval of time by Drosophila melanogaster larvae exposed to diets of different macronutrient quality. These assays are conducted in the context of a neuronal thermogenetic screen.

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