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Ensayo de inmunoprecipitación de ARN metilado para estudiar la modificación m⁵C en Arabidopsis

JoVE Journal
Genética

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JoVE Journal Genética

Methylated RNA Immunoprecipitation Assay to Study m⁵C Modification in Arabidopsis

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Ensayo de inmunoprecipitación de ARN metilado para estudiar la modificación m⁵C en Arabidopsis

6,048 Views

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08:50 min

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May 14, 2020

DOI:

10.3791/61231-v

DOI:

10.3791/61231-v

•

08:50 min

May 14, 2020

•

5 Views

Eleftheria Saplaoura*¹, Valentina Perrera*², Vincent Colot, Friedrich Kragler

¹Max Planck Institute of Molecular Plant Physiology, ²Department of Molecular Medicine, Medical School,University of Padua, ³Biologie de l’Ecole Normale Supérieure (IBENS), Paris, France

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Methylated RNA Immunoprecipitation MeRIP RNA Modification Transcriptome Arabidopsis RNA Extraction Guanidine Thiocyanate Acid Phenol 1-bromo-3-chloropropane Isopropanol Sodium Acetate Ethanol DNase RNA Integrity Number In Vitro Transcript Sonication

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