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Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques
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Neurociência
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JoVE Journal Neurociência
Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques

Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques

DOI:
10.3791/60965-v

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06:21 min

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May 07, 2020

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, , , ,
1Department of Medicine,University of Washington

Capítulos

  • 00:04Introduction
  • 00:46Preparing the Stereotax for Angled Procedure
  • 02:02Aligning the Central Axis of Rotation for Angled Procedure
  • 03:09Fiberoptic Implantation
  • 05:05Results: Representative Bilateral Ventromedial Hypothalamus Targeting
  • 05:44Conclusion

Summary

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Described here is a stereotactic procedure that can target challenging and difficult-to-reach brain regions (due to spatial limitations) using an angled coronal approach. This protocol is adaptable to both mouse and rat models and can be applied to diverse neuroscientific applications, including cannula implantation and microinjections of viral constructs.

Tags

Stereotactic ProcedureAdaptableMiceRatsNeuroscientific TechniquesBrain TargetingHead HolderMicromanipulatorCalibrationEar BarsCentering ScopeMedial-lateralAnterior-posteriorCoronal TiltAngled ImplantationCannulaDrillingMicroinjection

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