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Adaptable Angled Stereotactic Approach for Versatile Neuroscience Techniques
章
- 00:04Introduction
- 00:46Preparing the Stereotax for Angled Procedure
- 02:02Aligning the Central Axis of Rotation for Angled Procedure
- 03:09Fiberoptic Implantation
- 05:05Results: Representative Bilateral Ventromedial Hypothalamus Targeting
- 05:44Conclusion
Described here is a stereotactic procedure that can target challenging and difficult-to-reach brain regions (due to spatial limitations) using an angled coronal approach. This protocol is adaptable to both mouse and rat models and can be applied to diverse neuroscientific applications, including cannula implantation and microinjections of viral constructs.
