在无馈体和化学定义的培养条件下，使用人类多能干细胞的后多能感感神经元的有效分化

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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento

Efficient Differentiation of Postganglionic Sympathetic Neurons using Human Pluripotent Stem Cells under Feeder-free and Chemically Defined Culture Conditions

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在无馈体和化学定义的培养条件下，使用人类多能干细胞的后多能感感神经元的有效分化

DOI:

10.3791/60843-v

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10:24 min

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May 24, 2020

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Hsueh Fu Wu², Nadja Zeltner^2,3

¹Center for Molecular Medicine,University of Georgia, ²Department of Biochemistry and Molecular Biology, Franklin College of Arts and Sciences,University of Georgia, ³Department of Cellular Biology, Franklin College of Arts and Sciences,University of Georgia

Capítulos

00:04Introduction
00:50Human Pluripotent Stem Cell (hPSC) Maintenance
01:48hPSC Differentiation (Day 0)
03:34Neural Crest Cell (NCC) Induction (Day 1-10)
04:02NC Spheroid Aggregation (Day 10)
05:14NC Spheroid Maintenance and Sympathetic Progenitor Induction (Day 11-14)
06:09Sympathetic Neuron (symN) Differentiation and Maturation (Day 14-20 or 28-35)
07:38Results: Representative NC Induction, Maintenance, and Expansion
09:43Conclusion

Summary

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在此协议中，我们描述了一种稳定、高效的分化策略，用于从人类多能干细胞生成后强感致感神经元。此模型将使神经元可用于多个自主障碍的研究。

在无馈体和化学定义的培养条件下，使用人类多能干细胞的后多能感感神经元的有效分化

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