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质谱导览基因组挖掘作为发现新天然产品的工具
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JoVE Journal Química
Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
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质谱导览基因组挖掘作为发现新天然产品的工具

DOI:
10.3791/60825-v

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11:13 min

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March 12, 2020

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, , ,
1Department of Organic Chemistry, Institute of Chemistry,University of Campinas (UNICAMP), 2Center for Natural and Human Sciences,Federal University of ABC (UFABC)

Capítulos

  • 00:04Introduction
  • 02:54Genome Mining for Biosynthetic Gene Clusters
  • 03:46Strain Cultivation
  • 04:51Acquiring Mass Spectra and Preparation for GNPS Analysis
  • 05:43GNPS Analysis
  • 08:33Results: Genome Sequence Analysis and Molecular Networking
  • 09:46Conclusion

Summary

Tadução automática

Tadução automática

这里建立了一个质谱仪指导基因组挖掘协议并对此进行描述。它基于基因组序列信息和LC-MS/MS分析，旨在促进从复杂的微生物和植物提取物的分子的识别。

Tags

Mass SpectrometryGenome MiningNatural Product DiscoveryMolecular NetworkingWhole Genome SequencingGenotype-to-chemotypeBiosynthetic ClustersSecondary MetabolismGNPSStreptomycesFungiPlants

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