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Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products
JoVE 杂志
化学
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JoVE 杂志 化学
Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

Mass Spectrometry-Guided Genome Mining as a Tool to Uncover Novel Natural Products

DOI:
10.3791/60825-v

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11:13 min

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March 12, 2020

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, , ,
1Department of Organic Chemistry, Institute of Chemistry,University of Campinas (UNICAMP), 2Center for Natural and Human Sciences,Federal University of ABC (UFABC)

Chapters

  • 00:04Introduction
  • 02:54Genome Mining for Biosynthetic Gene Clusters
  • 03:46Strain Cultivation
  • 04:51Acquiring Mass Spectra and Preparation for GNPS Analysis
  • 05:43GNPS Analysis
  • 08:33Results: Genome Sequence Analysis and Molecular Networking
  • 09:46Conclusion

Summary

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A mass spectrometry-guided genome mining protocol is established and described here. It is based on genome sequence information and LC-MS/MS analysis and aims to facilitate identification of molecules from complex microbial and plant extracts.

Tags

Mass SpectrometryGenome MiningNatural Product DiscoveryMolecular NetworkingWhole Genome SequencingGenotype-to-chemotypeBiosynthetic ClustersSecondary MetabolismGNPSStreptomycesFungiPlants

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