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Biologia do Desenvolvimento

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全実装免疫学化学と3D再構成を用いた咽頭弓動脈の可視化と解析

JoVE Journal
Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Visualization and Analysis of Pharyngeal Arch Arteries using Whole-mount Immunohistochemistry and 3D Reconstruction

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全実装免疫学化学と3D再構成を用いた咽頭弓動脈の可視化と解析

9,276 Views

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10:02 min

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March 31, 2020

DOI:

10.3791/60797-v

DOI:

10.3791/60797-v

•

10:02 min

March 31, 2020

•

11 Views

AnnJosette Ramirez², Sophie Astrof

¹Department of Cell Biology and Molecular Medicine, New Jersey Medical School,Rutgers Biomedical and Health Sciences, ²Multidisciplinary PhD Program in Biomedical Sciences: Cell Biology, Neuroscience and Physiology Track, New Jersey Medical School,Rutgers Biomedical and Health Sciences

Tags

Visualization Analysis Pharyngeal Arch Arteries Whole-mount Immunohistochemistry 3D Reconstruction Endothelial Cell Quantification Aortic Arch Arteries Congenital Heart Disease Vessel Formation Remodeling Light-sheet Microscopy Mouse Embryo Fixation Permeabilization Blocking Buffer Primary Antibody Secondary Antibody Plastic Paraffin Molds

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