JoVE Journal
Biochemistry
Biochemistry
This Conteúdo is Open Access.
Capítulos
Resumo
This report describes screening of different detergents for preparing the visual GPCR, rhodopsin, and its complex with mini-Go. Biochemical methods characterizing the quality of the complex at different stages during purification are demonstrated. This protocol can be generalized to other membrane protein complexes for their future structural studies.