Journal
/
Bioquímica
/
Strategisk screening og karakterisering av Visual GPCR-mini-G Protein Signaling Complex for vellykket krystallisering
JoVE Journal
Bioquímica
This content is Free Access.
JoVE Journal Bioquímica
Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization

Strategisk screening og karakterisering av Visual GPCR-mini-G Protein Signaling Complex for vellykket krystallisering

6,618 Views

•

09:19 min

•

March 16, 2020

DOI:

10.3791/60747-v

DOI:
10.3791/60747-v

•

09:19 min
March 16, 2020

•

2 Views
, , , , , ,
1Laboratory of Biomolecular Research,Paul Scherrer Institute, 2Department of Biology,ETH Zürich, 3Center for Radiopharmaceutical Sciences,Paul Scherrer Institute, 4Laboratory of Molecular Biology,Medical Research Council

Tags

GPCRMini-G ProteinSignal TransductionMembrane Protein ComplexDetergent ScreeningCrystallizationHEK293 CellsAffinity Purification9-cis RetinalBuffer Exchange

Read Article

Cite this Article

Pamula, F., Mühle, J., Blanc, A., Nehmé, R., Edwards, P. C., Tate, C. G., Tsai, C. Strategic Screening and Characterization of the Visual GPCR-mini-G Protein Signaling Complex for Successful Crystallization. J. Vis. Exp. (157), e60747, doi:10.3791/60747 (2020).

Download .ris file

Copy

Share Video

.

Copy