Journal
/
Imunologia e Infecção
/
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

Localization and Quantification of Begomoviruses in Whitefly Tissues by Immunofluorescence and Quantitative PCR

DOI:
10.3791/60731-v

•

06:25 min

•

February 08, 2020

•

, , , , ,
1Ministry of Agriculture Key Laboratory of Molecular Biology of Crop Pathogen and Insects, Institute of Insect Sciences,Zhejiang University

Capítulos

  • 00:05Introduction
  • 00:43Female Whitefly Midgut, Primary Salivary Gland (PSG), Ovary, and Hemolymph Dissection
  • 02:03Tomato Yellow Leaf Curl Virus (TYLCV) Localization
  • 03:40TYLCV Quantification
  • 05:04Results: Representative TYLCV Detection and Quantification
  • 05:46Conclusion

Summary

Tadução automática

Tadução automática

We describe an immunofluorescence and quantitative PCR method for the localization and quantification of begomoviruses in insect tissues. The immunofluorescence protocol can be used to colocalize viral and vector proteins. The quantitative PCR protocol can be extended to quantify viruses in whole whitefly bodies and virus-infected plants.

Tags

BegomovirusesWhiteflyLocalizationQuantificationImmunofluorescenceQuantitative PCRVirus-vector InteractionVirus DetectionVirus TransmissionMicroscopyVirus Quantification

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Detection of MicroRNAs in Microglia by Real-time PCR in Normal CNS and During Neuroinflammation

Fluorescence in situ Hybridizations (FISH) for the Localization of Viruses and Endosymbiotic Bacteria in Plant and Insect Tissues

Simultaneous Quantification of T-Cell Receptor Excision Circles (TRECs) and K-Deleting Recombination Excision Circles (KRECs) by Real-time PCR

Development and Validation of a Quantitative PCR Method for Equid Herpesvirus-2 Diagnostics in Respiratory Fluids

Genotyping of Staphylococcus aureus by Ribosomal Spacer PCR (RS-PCR)

High-throughput Detection of Respiratory Pathogens in Animal Specimens by Nanoscale PCR

Metabolic Mapping: Quantitative Enzyme Cytochemistry and Histochemistry to Determine the Activity of Dehydrogenases in Cells and Tissues

Quantification of Efferocytosis by Single-cell Fluorescence Microscopy

Quantitative PCR of T7 Bacteriophage from Biopanning

Induction and Scoring of Graft-Versus-Host Disease in a Xenogeneic Murine Model and Quantification of Human T Cells in Mouse Tissues using Digital PCR

Read Article