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应用活细胞成像和低温电子断层扫描解决军团菌肺瘤点/Icm分泌系统的时空特征
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Applying Live Cell Imaging and Cryo-Electron Tomography to Resolve Spatiotemporal Features of the Legionella pneumophila Dot/Icm Secretion System
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应用活细胞成像和低温电子断层扫描解决军团菌肺瘤点/Icm分泌系统的时空特征

DOI:
10.3791/60693-v

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09:12 min

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March 10, 2020

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, , , ,
1Department of Microbial Pathogenesis, Boyer Center for Molecular Medicine,Yale University School of Medicine, 2Microbial Sciences Institute,Yale University, 3Department of Microbiology and Molecular Genetics, McGovern Medical School,The University of Texas Health Science Center at Houston

Capítulos

  • 00:04Introduction
  • 00:55Live Cell Imaging of L. pneumophila with Fluorescently Tagged Dot/Icm Components
  • 02:47Quantification of Polar Localization and Dynamics of Dot/Icm Components
  • 05:36Detection of sfGFP Mass Density with Cryo-ET
  • 06:23Results: Imaging Dot/Icm Secretion Systems in Intact Bacteria
  • 08:17Conclusion

Summary

Tadução automática

Tadução automática

细菌细胞成像是一种新兴的系统生物学方法，侧重于定义静态和动态过程，这些过程决定了大型大分子机器的功能。在这里，定量活细胞成像和低温电子断层扫描的集成用于研究军团菌肺瘤IV型分泌系统架构和功能。

Tags

Keywords: Live Cell ImagingCryo-electron TomographyLegionella PneumophilaDot/Icm Secretion SystemBacterial Secretion ComplexesHost-pathogen InteractionsStructural-functional RelationshipsAgarose PadsFluorescence MicroscopyPolarity Quantification

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