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15:04 min
November 02, 2019
DOI:
10.3791/60661-v
我们提出了一种方法,结合膜蛋白纯化和重组到消化片在一个单一的色谱步骤。生物素氨酸脚手架用于通过生物层干涉测量直接表面附着和蛋白质-配体相互作用的测量。
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Saville, J. W., Troman, L. A., Duong Van Hoa, F. PeptiQuick, a One-Step Incorporation of Membrane Proteins into Biotinylated Peptidiscs for Streamlined Protein Binding Assays. J. Vis. Exp. (153), e60661, doi:10.3791/60661 (2019).
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