Journal
/
Imunologia e Infecção
/
在 Vivo 感染利什曼病亚马孙病评估小鼠的寄生虫毒性
JoVE Journal
Imunologia e Infecção
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Imunologia e Infecção
In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice
Please note that all translations are automatically generated. Click here for the English version.

在 Vivo 感染利什曼病亚马孙病评估小鼠的寄生虫毒性

DOI:
10.3791/60617-v

•

06:57 min

•

February 20, 2020

•

, , , ,
1Department of Physiology, Institute of Bioscience,University of Sao Paulo

Capítulos

  • 00:04Introduction
  • 01:16In Vitro Differentiation of L. amazonensis Promastigotes into Axenic Amastigotes
  • 01:56C57BL/6 Footpad Infection with L. amazonensis
  • 02:47Mouse Footpad Lesion Extraction and Parasite Load Determination
  • 04:29Results: LIR1 Knockout In Vivo Lesion Development and Intracellular Replication
  • 06:03Conclusion

Summary

Tadução automática

Tadução automática

在这里，我们提出了一个编译的协议，以评估小鼠与利什曼病的皮感染。这是研究寄生虫毒性的可靠方法，允许对脊椎动物宿主对感染的反应进行系统观察。

Tags

Leishmania AmazonensisIn Vivo InfectionVirulenceCutaneous LeishmaniasisMouse ModelPromastigotesAmastigotesSubplantar InjectionLesion ProgressionParasite Quantification

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

在Live主机转利什曼原虫寄生虫在体内成像

γHV68感染小鼠的测量

使用人工痰介质测试抗生素药效试验<em>绿脓杆菌</em>更多相关的肺囊肿性纤维化的条件

Antileishmanial筛选对细胞内的寄生虫救援和转化实验<em>杜氏利什曼原虫</em无鞭毛体在THP1人急性单核细胞白血病细胞株

中性粒细胞分离和分析，以确定他们的淋巴瘤细胞的敏感性对治疗药物的作用

巨噬细胞内细胞内生长的定量化是评价利什曼原虫寄生虫毒力的快速可靠方法

线虫模型中气单胞菌感染的毒力及发病机制评价

精确的大脑映射，在小鼠中执行神经-免疫动力学的重复体内振动成像

前体内分析研究胃 肠道的 念珠菌血脂磷

非编码小RNA MicC有助于肠炎 沙门氏菌 外膜蛋白的毒力

Read Article