Rivista
/
Immunologia e infezioni
/
In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice
JoVE Journal
Immunologia e infezioni
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Immunologia e infezioni
In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

In Vivo Infection with Leishmania amazonensis to Evaluate Parasite Virulence in Mice

DOI:
10.3791/60617-v

•

06:57 min

•

February 20, 2020

•

, , , ,
1Department of Physiology, Institute of Bioscience,University of Sao Paulo

Capitoli

  • 00:04Introduction
  • 01:16In Vitro Differentiation of L. amazonensis Promastigotes into Axenic Amastigotes
  • 01:56C57BL/6 Footpad Infection with L. amazonensis
  • 02:47Mouse Footpad Lesion Extraction and Parasite Load Determination
  • 04:29Results: LIR1 Knockout In Vivo Lesion Development and Intracellular Replication
  • 06:03Conclusion

Summary

Traduzione automatica

Traduzione automatica

Here, we present a compiled protocol to evaluate the cutaneous infection of mice with Leishmania amazonensis. This is a reliable method for studying parasite virulence, allowing a systemic view of the vertebrate host response to the infection.

Tags

Leishmania AmazonensisIn Vivo InfectionVirulenceCutaneous LeishmaniasisMouse ModelPromastigotesAmastigotesSubplantar InjectionLesion ProgressionParasite Quantification

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

In vivo Imaging of Transgenic Leishmania Parasites in a Live Host

Measurement of γHV68 Infection in Mice

Use of Artificial Sputum Medium to Test Antibiotic Efficacy Against Pseudomonas aeruginosa in Conditions More Relevant to the Cystic Fibrosis Lung

A Parasite Rescue and Transformation Assay for Antileishmanial Screening Against Intracellular Leishmania donovani Amastigotes in THP1 Human Acute Monocytic Leukemia Cell Line

Neutrophil Isolation and Analysis to Determine their Role in Lymphoma Cell Sensitivity to Therapeutic Agents

Quantification of Intracellular Growth Inside Macrophages is a Fast and Reliable Method for Assessing the Virulence of Leishmania Parasites

Evaluating Virulence and Pathogenesis of Aeromonas Infection in a Caenorhabditis elegans Model

Precise Brain Mapping to Perform Repetitive In Vivo Imaging of Neuro-Immune Dynamics in Mice

An Ex vivo Assay to Study Candida albicans Hyphal Morphogenesis in the Gastrointestinal Tract

A Non-Coding Small RNA MicC Contributes to Virulence in Outer Membrane Proteins in Salmonella Enteritidis

Read Article