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通过基因表达分析的改良方法从谷物种子获取高质量的转录数据
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Obtaining High-Quality Transcriptome Data from Cereal Seeds by a Modified Method for Gene Expression Profiling
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通过基因表达分析的改良方法从谷物种子获取高质量的转录数据

DOI:
10.3791/60602-v

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07:18 min

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May 21, 2020

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, , ,
1Department of Chemistry and Biotechnology,Swinburne University of Technology, 2Department of Molecular Genetics, Heterosis Research Group,Leibniz Institute of Plant Genetics and Crop Plant Research (IPK), 3Grain Quality and Nutrition Center, Applied Functional Genomics Cluster,International Rice Research Institute

Capítulos

  • 00:04Introduction
  • 00:40Microarray Hybridization
  • 03:09Microarray Scanning
  • 05:05Results: Representative Transcriptome Data Analyses
  • 06:39Conclusion

Summary

Tadução automática

Tadução automática

提出了谷物转录分析的方法。基于微阵列的基因表达分析从谷物中分离高质量的总RNA开始，并随着cDNA的生成而继续。在cRNA标签和微阵列杂交后，提出了信号检测和质量控制的建议。

Tags

Microarray HybridizationGene Expression ProfilingCereal SeedsTranscriptome DataHigh-throughput SequencingBlocking AgentFragmentation MixHybridization BufferMicroarray SlideHybridization AssemblyHybridization Oven

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