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Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
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Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
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April 10, 2020
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Cell EnvelopeGram-negative BacteriaInner MembraneOuter MembraneLipidsProteinsSugarsAntibiotic KillingAntimicrobial ResistanceDisease PathogenesisAcinetobacter BaumanniiCell FractionationDetergent-freeSemi-native EnvironmentOptical DensityCentrifugationLysozymeEDTAProtease InhibitorMagnesium ChlorideNuclease
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Cian, M. B., Giordano, N. P., Mettlach, J. A., Minor, K. E., Dalebroux, Z. D. Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii. J. Vis. Exp. (158), e60517, doi:10.3791/60517 (2020).
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