Journal
/
Immunologie und Infektion
/
Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
JoVE Journal
Immunologie und Infektion
Zum Anzeigen dieser Inhalte ist ein JoVE-Abonnement erforderlich.  Melden Sie sich an oder starten Sie Ihre kostenlose Testversion.
JoVE Journal Immunologie und Infektion
Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii

DOI:
10.3791/60517-v

•

10:24 min

•

April 10, 2020

•

, , , ,
1Department of Microbiology and Immunology,University of Oklahoma Health Sciences Center

Kapitel

  • 00:04Introduction
  • 00:55Preparation of Bacteria for Membrane Extraction
  • 02:18Dissociation of the Outer Membrane and Plasmolysis
  • 03:56Pressurized Homogenization and Lysis
  • 04:35Total Membrane Fractionation
  • 05:34Separating the Dual Membranes
  • 08:00Results: Purity and Quality of Inner and Outer Membrane Fractions
  • 09:36Conclusion

Summary

Automatische Übersetzung

Automatische Übersetzung

Gram-negative bacteria produce two spatially segregated membranes. The outer membrane is partitioned from the inner membrane by a periplasm and a peptidoglycan layer. The ability to isolate the dual bilayers of these microbes has been critical for understanding their physiology and pathogenesis.

Tags

Cell EnvelopeGram-negative BacteriaInner MembraneOuter MembraneLipidsProteinsSugarsAntibiotic KillingAntimicrobial ResistanceDisease PathogenesisAcinetobacter BaumanniiCell FractionationDetergent-freeSemi-native EnvironmentOptical DensityCentrifugationLysozymeEDTAProtease InhibitorMagnesium ChlorideNuclease

Article

Embed

ZUR WIEDERGABELISTE HINZUFÜGEN

Usage Statistics

Verwandte Videos

Determination of Molecular Structures of HIV Envelope Glycoproteins using Cryo-Electron Tomography and Automated Sub-tomogram Averaging

Purification and Visualization of Lipopolysaccharide from Gram-negative Bacteria by Hot Aqueous-phenol Extraction

Tractable Mammalian Cell Infections with Protozoan-primed Bacteria

Fluorescence Microscopy Methods for Determining the Viability of Bacteria in Association with Mammalian Cells

Rapid Identification of Gram Negative Bacteria from Blood Culture Broth Using MALDI-TOF Mass Spectrometry

In Vivo Investigation of Antimicrobial Blue Light Therapy for Multidrug-resistant Acinetobacter baumannii Burn Infections Using Bioluminescence Imaging

Legionella pneumophila Outer Membrane Vesicles: Isolation and Analysis of Their Pro-inflammatory Potential on Macrophages

Application of Consistent Massage-Like Perturbations on Mouse Calves and Monitoring the Resulting Intramuscular Pressure Changes

Separation of Rat Epidermis and Dermis with Thermolysin to Detect Site-Specific Inflammatory mRNA and Protein

A Neonatal Imaging Model of Gram-Negative Bacterial Sepsis

Read Article