/
/
Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii
12,545 Views
•
10:24 min
•
April 10, 2020
DOI:
Summary
Automatically generated
Gram-negative bacteria produce two spatially segregated membranes. The outer membrane is partitioned from the inner membrane by a periplasm and a peptidoglycan layer. The ability to isolate the dual bilayers of these microbes has been critical for understanding their physiology and pathogenesis.
Explore More Videos
Cell EnvelopeGram-negative BacteriaInner MembraneOuter MembraneLipidsProteinsSugarsAntibiotic KillingAntimicrobial ResistanceDisease PathogenesisAcinetobacter BaumanniiCell FractionationDetergent-freeSemi-native EnvironmentOptical DensityCentrifugationLysozymeEDTAProtease InhibitorMagnesium ChlorideNuclease
Read Article
Cite this Article
Cian, M. B., Giordano, N. P., Mettlach, J. A., Minor, K. E., Dalebroux, Z. D. Separation of the Cell Envelope for Gram-negative Bacteria into Inner and Outer Membrane Fractions with Technical Adjustments for Acinetobacter baumannii. J. Vis. Exp. (158), e60517, doi:10.3791/60517 (2020).
Copy