Journal
/
Neurociência
/
Microinjectrode System for Combined Drug Infusion and Electrophysiology
JoVE Journal
Neurociência
This content is Free Access.
JoVE Journal Neurociência
Microinjectrode System for Combined Drug Infusion and Electrophysiology

Microinjectrode System for Combined Drug Infusion and Electrophysiology

DOI:
10.3791/60365-v

•

08:30 min

•

November 13, 2019

•

, , ,
1Department of Ophthalmology and Visual Sciences,University of Utah, 2Department of Biomedical Engineering,University of Utah, 3Department of Electrical Engineering and Computer Science,University of California, Irvine, 4Department of Biomedical Engineering,University of California, Irvine

Capítulos

  • 00:04Título
  • 00:46Construction of the Microinjectrode for Stimulation and Recording
  • 02:21Microfluidic System
  • 03:22Preparation of the Microfluidic System
  • 05:46Performing Recording for an Infusion Experiment
  • 06:38Results: Memory Guided Saccade Task during Muscimol Infusion in FEF (Frontal Eye Field)
  • 07:26Conclusion

Summary

Tadução automática

Tadução automática

We present a microinjectrode system designed for electrophysiology and assisted delivery of experimental probes (i.e., nanosensors, microelectrodes), with optional drug infusion. Widely available microfluidic components are coupled to a cannula containing the probe. A step-by-step protocol for microinjectrode construction is included, with results during muscimol infusion in macaque cortex.

Tags

Microinjectrode SystemDrug InfusionElectrophysiologyMicroelectrodesNanosensorsAwake Behaving AnimalsMicrofluidic InfusionNanoliter ScaleCannulaT JunctionCapillary TubingGold PinEpoxy GlueThree-way ValvesMicrofluidic Circuit

Article

Embed

ADICIONAR À PLAYLIST

Usage Statistics

Vídeos Relacionados

Simultaneous fMRI and Electrophysiology in the Rodent Brain

Systemic and Local Drug Delivery for Treating Diseases of the Central Nervous System in Rodent Models

Novel Apparatus and Method for Drug Reinforcement

Spinal Cord Electrophysiology II: Extracellular Suction Electrode Fabrication

Culturing and Electrophysiology of Cells on NRCC Patch-clamp Chips

Paired Nanoinjection and Electrophysiology Assay to Screen for Bioactivity of Compounds using the Drosophila melanogaster Giant Fiber System

Stereotaxic Infusion of Oligomeric Amyloid-beta into the Mouse Hippocampus

Prolonged Incubation of Acute Neuronal Tissue for Electrophysiology and Calcium-imaging

A Computerized Test Battery to Study Pharmacodynamic Effects on the Central Nervous System of Cholinergic Drugs in Early Phase Drug Development

Combined Transcranial Magnetic Stimulation and Electroencephalography of the Dorsolateral Prefrontal Cortex

Read Article