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Microinjectrode System for Combined Drug Infusion and Electrophysiology
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Neurosciences
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Journal JoVE Neurosciences
Microinjectrode System for Combined Drug Infusion and Electrophysiology

Microinjectrode System for Combined Drug Infusion and Electrophysiology

6,470 Views

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08:30 min

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November 13, 2019

DOI:

10.3791/60365-v

DOI:
10.3791/60365-v

•

08:30 min
November 13, 2019

•

1 Views
, , ,
1Department of Ophthalmology and Visual Sciences,University of Utah, 2Department of Biomedical Engineering,University of Utah, 3Department of Electrical Engineering and Computer Science,University of California, Irvine, 4Department of Biomedical Engineering,University of California, Irvine

Summary

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We present a microinjectrode system designed for electrophysiology and assisted delivery of experimental probes (i.e., nanosensors, microelectrodes), with optional drug infusion. Widely available microfluidic components are coupled to a cannula containing the probe. A step-by-step protocol for microinjectrode construction is included, with results during muscimol infusion in macaque cortex.

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Cite this Article

Vanegas, M. I., Hubbard, K. R., Esfandyarpour, R., Noudoost, B. Microinjectrode System for Combined Drug Infusion and Electrophysiology. J. Vis. Exp. (153), e60365, doi:10.3791/60365 (2019).

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