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Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

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08:34 min

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January 08, 2020

DOI:

10.3791/60297-v

DOI:
10.3791/60297-v

•

08:34 min
January 08, 2020

•

1 Views
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1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics,Joan C. Edwards School of Medicine at Marshall University

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Pseudomonas AeruginosaIn-frame Gene DeletionVirulence AttenuationMouse Infection ModelReproducibilityCrossover RecombinantColony PCRDouble Crossover RecombinantCarbenicillin SensitivitySucrose ResistanceGene Mutation

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Valentine, M. E., Kirby, B. D., Yu, H. D. Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection. J. Vis. Exp. (155), e60297, doi:10.3791/60297 (2020).

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