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Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
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JoVE Journal 免疫学と感染
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

DOI:
10.3791/60297-v

•

08:34 min

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January 08, 2020

•

, ,
1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics,Joan C. Edwards School of Medicine at Marshall University

章

  • 00:05標題
  • 01:24Detection of Double-crossover Recombinants of P. aeruginosa
  • 02:24Confirmation of Gene Deletion with Colony PCR
  • 03:07Visualization of Bioluminescence-labeled Bacteria in Mice
  • 05:42Inoculation of Animals with Bacterial Strains
  • 06:28Results: Evaluation of Attenuated P. aeruginosa Strain
  • 07:24Conclusion

概要

自動翻訳

自動翻訳

Here, we describe a simple and reproducible protocol of mouse model of infection to evaluate the attenuation of the genetically modified strains of Pseudomonas aeruginosa in comparison to the United States Food and Drug Administration (FDA)-approved Escherichia coli for commercial applications.

タグ

Pseudomonas AeruginosaIn-frame Gene DeletionVirulence AttenuationMouse Infection ModelReproducibilityCrossover RecombinantColony PCRDouble Crossover RecombinantCarbenicillin SensitivitySucrose ResistanceGene Mutation

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