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Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
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JoVE 杂志 免疫与感染
Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection

6,480 Views

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08:34 min

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January 08, 2020

DOI:

10.3791/60297-v

DOI:
10.3791/60297-v

•

08:34 min
January 08, 2020

•

3 Views
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1Progenesis Technologies, LLC, 2Department of Biomedical Sciences, Pediatrics,Joan C. Edwards School of Medicine at Marshall University

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Here, we describe a simple and reproducible protocol of mouse model of infection to evaluate the attenuation of the genetically modified strains of Pseudomonas aeruginosa in comparison to the United States Food and Drug Administration (FDA)-approved Escherichia coli for commercial applications.

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Pseudomonas AeruginosaIn-frame Gene DeletionVirulence AttenuationMouse Infection ModelReproducibilityCrossover RecombinantColony PCRDouble Crossover RecombinantCarbenicillin SensitivitySucrose ResistanceGene Mutation

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Valentine, M. E., Kirby, B. D., Yu, H. D. Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection. J. Vis. Exp. (155), e60297, doi:10.3791/60297 (2020).

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