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Generation of In-Frame Gene Deletion Mutants in Pseudomonas aeruginosa and Testing for Virulence Attenuation in a Simple Mouse Model of Infection
Chapters
- 00:05Title
- 01:24Detection of Double-crossover Recombinants of P. aeruginosa
- 02:24Confirmation of Gene Deletion with Colony PCR
- 03:07Visualization of Bioluminescence-labeled Bacteria in Mice
- 05:42Inoculation of Animals with Bacterial Strains
- 06:28Results: Evaluation of Attenuated P. aeruginosa Strain
- 07:24Conclusion
Here, we describe a simple and reproducible protocol of mouse model of infection to evaluate the attenuation of the genetically modified strains of Pseudomonas aeruginosa in comparison to the United States Food and Drug Administration (FDA)-approved Escherichia coli for commercial applications.
