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使用路西法黄电磷学可视化星形形态
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Neurociência
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JoVE Journal Neurociência
Visualizing Astrocyte Morphology Using Lucifer Yellow Iontophoresis
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使用路西法黄电磷学可视化星形形态

DOI:
10.3791/60225-v

•

07:38 min

•

September 14, 2019

•

, , ,
1Department of Physiology, David Geffen School of Medicine,University of California Los Angeles, 2Department of Neurobiology, David Geffen School of Medicine,University of California Los Angeles

Capítulos

  • 00:04Título
  • 00:31Electrode Preparation
  • 01:25DYE Ejection Test
  • 02:44Astrocyte Filling
  • 04:24Imaging of Filled Astrocyte
  • 05:15Results: Visualization of Astrocyte Morphology
  • 06:56Conclusion

Summary

Tadução automática

Tadução automática

星形细胞是形态复杂的细胞，其多重过程和茂密的区域就是例证。为了分析它们的精细形态，我们提出了一种可靠的方案，用于在轻度固定组织中执行细胞内路西法黄色淋病。

Tags

Keywords: Astrocyte MorphologyLucifer Yellow IontophoresisAstrocyte-neuronal InteractionsSynapseBrain SliceConfocal MicroscopyDye EjectionAstrocyte IdentificationStratum RadiatumHippocampus

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