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Elektroporation methode voor in vivo levering van plasmide DNA in de volwassen zebravis Telencephalon
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Biologia do Desenvolvimento
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JoVE Journal Biologia do Desenvolvimento
Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon
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Elektroporation methode voor in vivo levering van plasmide DNA in de volwassen zebravis Telencephalon

9,441 Views

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06:02 min

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September 13, 2019

DOI:

10.3791/60066-v

DOI:
10.3791/60066-v

•

06:02 min
September 13, 2019

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4 Views
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1Institute of Stem Cell Research,Helmholtz Zentrum München, 2Graduate School of Systemic Neurosciences,Ludwig Maximilian University, 3Department of Cell Biology and Anatomy, Biomedical Center,Ludwig Maximilian University, 4Munich Cluster for Systems Neurology (SyNergy)

Summary

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Hier is een elektroporation methode voor plasmide DNA levering en ependymoglial cellabeling in de volwassen zebravis telencephalon. Dit protocol is een snelle en efficiënte methode om individuele ependymoglial cellen te visualiseren en te traceren en opent nieuwe mogelijkheden om elektroporation toe te passen op een breed scala aan genetische manipulaties.

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Durovic, T., Ninkovic, J. Electroporation Method for In Vivo Delivery of Plasmid DNA in the Adult Zebrafish Telencephalon. J. Vis. Exp. (151), e60066, doi:10.3791/60066 (2019).

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