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用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应
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Bioquímica
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JoVE Journal Bioquímica
A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
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用于筛选小分子抑制剂的NADH耦合ATPase分析的半高通量自适应

DOI:
10.3791/60017-v

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10:28 min

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August 17, 2019

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, , , ,
1Department of Molecular Medicine,The Scripps Research Institute, 2Department of Neuroscience,The Scripps Research Institute, 3Laboratory of Molecular Physiology, NHLBI,National Institutes of Health

Capítulos

  • 00:04Título
  • 00:46Measuring ATPase Activities and Inhibitory Effects of Small Molecule Inhibitors
  • 05:48Analyzing Data
  • 07:54Results: Fluorescence Intensity
  • 09:26Conclusion

Summary

Tadução automática

Tadução automática

一种烟酰胺腺苷二核苷酸（NADH）与ATPase的检测已应用于小分子肌苷抑制剂的半高通量筛选。此动力学测定以 384 孔微孔板格式运行，总反应体积仅为每孔 20 μL。该平台应适用于几乎任何ADP生产酶。

Tags

ATPase AssayNADH-coupledSemi-high-throughput ScreeningActinMyosin IIEnzyme InhibitorsCalibrationPositive And Negative Controls

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