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A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
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A Semi-High-Throughput Adaptation of the NADH-Coupled ATPase Assay for Screening Small Molecule Inhibitors
Capítulos
- 00:04Título
- 00:46Measuring ATPase Activities and Inhibitory Effects of Small Molecule Inhibitors
- 05:48Analyzing Data
- 07:54Results: Fluorescence Intensity
- 09:26Conclusion
A nicotinamide adenine dinucleotide (NADH)-coupled ATPase assay has been adapted to semihigh throughput screening of small molecule myosin inhibitors. This kinetic assay is run in a 384-well microplate format with total reaction volumes of only 20 µL per well. The platform should be applicable to virtually any ADP producing enzyme.
