Journal
/
Neurociência
/
Une approche d'alimentation d'anticorps pour étudier le trafic de récepteurs de glutamate dans les cultures hippocampal primaires dissociées
JoVE Journal
Neurociência
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Neurociência
An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures
Please note that all translations are automatically generated. Click here for the English version.

Une approche d'alimentation d'anticorps pour étudier le trafic de récepteurs de glutamate dans les cultures hippocampal primaires dissociées

5,512 Views

•

08:13 min

•

August 02, 2019

DOI:

10.3791/59982-v

DOI:
10.3791/59982-v

•

08:13 min
August 02, 2019

•

1 Views
, , ,
1Department of Pharmacology, Feinberg School of Medicine,Northwestern University, 2Department of Cellular Neurophysiology,Institute of Physiology CAS

Tags

Antibody FeedingGlutamate Receptor TraffickingPrimary Hippocampal CulturesSurface ExpressionInternalizationRecyclingParaformaldehyde FixationFab Anti-IgGDynasore

Read Article

Cite this Article

Chiu, A. M., Barse, L., Hubalkova, P., Sanz-Clemente, A. An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures. J. Vis. Exp. (150), e59982, doi:10.3791/59982 (2019).

Download .ris file

Copy

Share Video

.

Copy