Waiting
Processando Login

Trial ends in Request Full Access Tell Your Colleague About Jove
Journal / Neuroscience / An Antibody Feeding Approach to Study Glutamate…
JoVE Journal
Neuroscience

É necessário ter uma assinatura JoVE para assistir este Faça login ou comece sua avaliação gratuita.

JoVE Journal Neuroscience
An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures
 

An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

Article DOI: 10.3791/59982-v • 08:13 min • August 2nd, 2019
August 2nd, 2019 •
1, 1, 1,2, 1
1Department of Pharmacology, Feinberg School of Medicine, Northwestern University, 2Department of Cellular Neurophysiology, Institute of Physiology CAS

Capítulos

Resumo
Automatic Translation

This article presents a method to study glutamate receptor (GluR) trafficking in dissociated primary hippocampal cultures. Using an antibody-feeding approach to label endogenous or overexpressed receptors in combination with pharmacological approaches, this method allows for the identification of molecular mechanisms regulating GluR surface expression by modulating internalization or recycling processes.

Tags

Antibody Feeding Glutamate Receptor Trafficking Dissociated Primary Hippocampal Cultures Surface Expressed Receptors Antibody Feeding Approach Internalization And Recycling Mechanistic Information Regulation Of Surface Expressed Proteins Glutamine Receptors Primary Hippocampal Neurons Anagenic Extracellular Epitope Tagged Constructs Endogenous GluA1 Tagged Glu12 B Cover Slips Conditioned Media Incubation And Washing Steps
Read Article

Get cutting-edge science videos from JoVE sent straight to your inbox every month.

Waiting X
Simple Hit Counter