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An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures
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Journal JoVE Neurosciences
An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

5,512 Views

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08:13 min

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August 02, 2019

DOI:

10.3791/59982-v

DOI:
10.3791/59982-v

•

08:13 min
August 02, 2019

•

1 Views
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1Department of Pharmacology, Feinberg School of Medicine,Northwestern University, 2Department of Cellular Neurophysiology,Institute of Physiology CAS

Tags

Antibody FeedingGlutamate Receptor TraffickingPrimary Hippocampal CulturesSurface ExpressionInternalizationRecyclingParaformaldehyde FixationFab Anti-IgGDynasore

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Chiu, A. M., Barse, L., Hubalkova, P., Sanz-Clemente, A. An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures. J. Vis. Exp. (150), e59982, doi:10.3791/59982 (2019).

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