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En antistof fodring tilgang til at studere glutamat receptor handel i dissocierede primære hippocampus kulturer
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Neurociência
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JoVE Journal Neurociência
An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures

En antistof fodring tilgang til at studere glutamat receptor handel i dissocierede primære hippocampus kulturer

5,512 Views

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08:13 min

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August 02, 2019

DOI:

10.3791/59982-v

DOI:
10.3791/59982-v

•

08:13 min
August 02, 2019

•

1 Views
, , ,
1Department of Pharmacology, Feinberg School of Medicine,Northwestern University, 2Department of Cellular Neurophysiology,Institute of Physiology CAS

Tags

Antibody FeedingGlutamate Receptor TraffickingPrimary Hippocampal CulturesSurface ExpressionInternalizationRecyclingParaformaldehyde FixationFab Anti-IgGDynasore

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Cite this Article

Chiu, A. M., Barse, L., Hubalkova, P., Sanz-Clemente, A. An Antibody Feeding Approach to Study Glutamate Receptor Trafficking in Dissociated Primary Hippocampal Cultures. J. Vis. Exp. (150), e59982, doi:10.3791/59982 (2019).

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