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Mappatura genetica delle differenze di termotolleranza tra le specie di Saccamici Yeast tramite l'analisi dell'emizigosità reciproca genomica
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Genética
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JoVE Journal Genética
Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis
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Mappatura genetica delle differenze di termotolleranza tra le specie di Saccamici Yeast tramite l'analisi dell'emizigosità reciproca genomica

15,469 Views

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10:08 min

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August 12, 2019

DOI:

10.3791/59972-v

DOI:
10.3791/59972-v

•

10:08 min
August 12, 2019

•

15 Views
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1Department of Plant and Microbial Biology,University of California Berkeley, 2Department of Biology,Stanford University, 3Buck Institute for Research on Aging

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Genetic MappingThermotoleranceSaccharomyces YeastGenome-wide Reciprocal Hemizygosity AnalysisHigh-throughputHigh ResolutionFreezer StockYPD AgarLiquid YPDOptical DensityBack DilutionTris-EDTA BufferLithium AcetatePolyethylene Glycol

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Weiss, C. V., Chuong, J. N., Brem, R. B. Genetic Mapping of Thermotolerance Differences Between Species of Saccharomyces Yeast via Genome-Wide Reciprocal Hemizygosity Analysis. J. Vis. Exp. (150), e59972, doi:10.3791/59972 (2019).

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