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原人类单核细胞的隔离、转染和文化
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Imunologia e Infecção
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JoVE Journal Imunologia e Infecção
Isolation, Transfection, and Culture of Primary Human Monocytes
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原人类单核细胞的隔离、转染和文化

12,648 Views

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09:13 min

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December 16, 2019

DOI:

10.3791/59967-v

DOI:
10.3791/59967-v

•

09:13 min
December 16, 2019

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3 Views
, , , , ,
1Department of Medicine,Louisiana State University Health Sciences Center, 2Department of Microbiology, Immunology, and Parasitology,Louisiana State University Health Sciences Center, 3Department of Biomedical and Clinical Sciences L. Sacco,University of Milan

Tags

Primary Human MonocytesIsolationTransfectionCultureHIV InfectionImmune DysfunctionAntiretroviral TherapyMolecular MechanismsBiosafetyEDTAMagnetic BeadsPBSCentrifugationCell Counting

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Plaisance-Bonstaff, K., Faia, C., Wyczechowska, D., Jeansonne, D., Vittori, C., Peruzzi, F. Isolation, Transfection, and Culture of Primary Human Monocytes. J. Vis. Exp. (154), e59967, doi:10.3791/59967 (2019).

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