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小鼠神经干细胞和脑中5-羟基甲基细胞氨酸的检测

JoVE Journal
Neurociência

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JoVE Journal Neurociência

The Detection of 5-Hydroxymethylcytosine in Neural Stem Cells and Brains of Mice

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小鼠神经干细胞和脑中5-羟基甲基细胞氨酸的检测

DOI:

10.3791/59950-v

•

08:03 min

•

September 19, 2019

•

Yingliang Zhuang², Junchen Chen², Weize Xu, Qiang Shu, Xuekun Li²

¹The Children’s Hospital, School of Medicine,Zhejiang University, ²The Institute of Translational Medicine, School of Medicine,Zhejiang University

Capítulos

00:04Título
01:00Transcardial Perfusion of the Mouse
02:03Brain Sectioning and Immunofluorescence Staining
04:02Genomic DNA Isolation
05:43DNA Dot Blot
07:03Results: Immunofluorescence Staining and Dot Blot Detection
07:49Conclusion

Summary

Tadução automática

在这里，我们提出了一个协议，利用免疫荧光染色和DNA点印法检测细胞和脑组织中5-羟基甲基细胞氨酸。

Tags

Keywords: 5-hydroxymethylcytosine 5hmC Immunofluorescence DNA Dot-blot Neural Stem Cells Mouse Brain Epigenetic Modification Gene Expression Neurological Disorders Perfusion Fixation Cryosectioning Immunostaining

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