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活联甘蔗，用于亲和色谱树脂的快速发展 - 抗体捕获作为案例研究
JoVE Journal
Bioquímica
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JoVE Journal Bioquímica
Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study
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活联甘蔗，用于亲和色谱树脂的快速发展 - 抗体捕获作为案例研究

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07:53 min

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August 16, 2019

DOI:

10.3791/59933-v

DOI:
10.3791/59933-v

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07:53 min
August 16, 2019

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1Institute for Molecular Biotechnology,RWTH Aachen University, 2Sanofi Deutschland GmbH, 3Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.

Tags

Activated Cross-linked AgaroseAffinity Chromatography ResinsAntibody CaptureMonoclonal AntibodiesProtein A ChromatographyAffinity LigandHIV Neutralizing Antibody 2F5Dynamic Binding CapacityResin ReusabilityPurification CostsNHS-Activated Cross-linked AgaroseCoupling Reaction

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Knödler, M., Rühl, C., Opdensteinen, P., Buyel, J. F. Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study. J. Vis. Exp. (150), e59933, doi:10.3791/59933 (2019).

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