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Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study
JoVE 杂志
生物化学
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JoVE 杂志 生物化学
Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study

8,939 Views

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07:53 min

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August 16, 2019

DOI:

10.3791/59933-v

DOI:
10.3791/59933-v

•

07:53 min
August 16, 2019

•

8 Views
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1Institute for Molecular Biotechnology,RWTH Aachen University, 2Sanofi Deutschland GmbH, 3Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.

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In this procedure, a DsRed-based epitope ligand is immobilized to produce a highly selective affinity resin for the capture of monoclonal antibodies from crude plant extracts or cell culture supernatants, as an alternative to Protein A.

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Knödler, M., Rühl, C., Opdensteinen, P., Buyel, J. F. Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study. J. Vis. Exp. (150), e59933, doi:10.3791/59933 (2019).

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