Journal
/
Biochimie
/
Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study
Journal JoVE
Biochimie
This content is Free Access.
Journal JoVE Biochimie
Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study

Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins – Antibody Capture as a Case Study

8,939 Views

•

07:53 min

•

August 16, 2019

DOI:

10.3791/59933-v

DOI:
10.3791/59933-v

•

07:53 min
August 16, 2019

•

8 Views
, , ,
1Institute for Molecular Biotechnology,RWTH Aachen University, 2Sanofi Deutschland GmbH, 3Fraunhofer Institute for Molecular Biology and Applied Ecology IME,Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e. V.

Summary

Automatically generated

In this procedure, a DsRed-based epitope ligand is immobilized to produce a highly selective affinity resin for the capture of monoclonal antibodies from crude plant extracts or cell culture supernatants, as an alternative to Protein A.

Explore More Videos

Activated Cross-linked AgaroseAffinity Chromatography ResinsAntibody CaptureMonoclonal AntibodiesProtein A ChromatographyAffinity LigandHIV Neutralizing Antibody 2F5Dynamic Binding CapacityResin ReusabilityPurification CostsNHS-Activated Cross-linked AgaroseCoupling Reaction

Read Article

Cite this Article

Knödler, M., Rühl, C., Opdensteinen, P., Buyel, J. F. Activated Cross-linked Agarose for the Rapid Development of Affinity Chromatography Resins - Antibody Capture as a Case Study. J. Vis. Exp. (150), e59933, doi:10.3791/59933 (2019).

Download .ris file

Copy

Share Video

.

Copy