We demonstrate a method to determine successful or failed fertilization on the basis of sperm nuclear morphology in Arabidopsis double fertilization using an epifluorescence microscope.
Takahashi, T., Igawa, T. Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization. J. Vis. Exp. (150), e59916, doi:10.3791/59916 (2019).