Rivista
/
Biologia dello sviluppo
/
Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization
JoVE Journal
Biologia dello sviluppo
È necessario avere un abbonamento a JoVE per visualizzare questo.  Accedi o inizia la tua prova gratuita.
JoVE Journal Biologia dello sviluppo
Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization

Evaluation of Fertilization State by Tracing Sperm Nuclear Morphology in Arabidopsis Double Fertilization

DOI:
10.3791/59916-v

•

00:05 min

•

August 29, 2019

•

,
1Graduate School of Horticulture,Chiba University

Capitoli

  • 00:05Titolo
  • 00:56Artificial Pollination
  • 02:03Ovule Observation
  • 03:24Results: Fertilization Phenotypes
  • 04:30Conclusion

Summary

Traduzione automatica

Traduzione automatica

We demonstrate a method to determine successful or failed fertilization on the basis of sperm nuclear morphology in Arabidopsis double fertilization using an epifluorescence microscope.

Tags

ArabidopsisDouble FertilizationSperm Nuclear MorphologyFertilization EvaluationHTR10-mRFPEmasculationPollinationOvuleFluorescence Microscopy

Article

Embed

AGGIUNGI ALLA PLAYLIST

Usage Statistics

Video correlati

Manipulation and In Vitro Maturation of Xenopus laevis Oocytes, Followed by Intracytoplasmic Sperm Injection, to Study Embryonic Development

Live Confocal Imaging of Developing Arabidopsis Flowers

Bottlenose Dolphin (Tursiops truncatus) Spermatozoa: Collection, Cryopreservation, and Heterologous In Vitro Fertilization

Analysis of Chromosome Segregation, Histone Acetylation, and Spindle Morphology in Horse Oocytes

A Method for Characterizing Embryogenesis in Arabidopsis

Live Cell Imaging of Chromosome Segregation During Mitosis

Tracing Gene Expression Through Detection of β-galactosidase Activity in Whole Mouse Embryos

Fluorimetric Techniques for the Assessment of Sperm Membranes

A Layered Mounting Method for Extended Time-Lapse Confocal Microscopy of Whole Zebrafish Embryos

Clonal Genetic Tracing using the Confetti Mouse to Study Mineralized Tissues

Read Article