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Redes de cardiomiocitos derivados de iPSC humanos en matrices de microelectrodos multipolos para grabaciones potenciales de acción recurrente
JoVE Journal
Bioengenharia
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JoVE Journal Bioengenharia
Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings
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Redes de cardiomiocitos derivados de iPSC humanos en matrices de microelectrodos multipolos para grabaciones potenciales de acción recurrente

11,143 Views

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08:53 min

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July 15, 2019

DOI:

10.3791/59906-v

DOI:
10.3791/59906-v

•

08:53 min
July 15, 2019

•

4 Views
, , , ,
1Aurora Research Institute,Advocate Aurora Health Care, 2Department of Biomedical Engineering, College of Engineering and Applied Science,University of Wisconsin-Milwaukee, 3Department of Medicine-Cardiovascular, School of Medicine,Johns Hopkins University

Summary

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Este artículo contiene un conjunto de protocolos para el desarrollo de redes de cardiomiocitos derivados de células madre pluripotentes inducidas por el hombre (hiPSC-CM) cultivadas en placas MEA multipocillos para electroportar la membrana celular de forma reversible para mediciones potenciales de acción. Las grabaciones de alto rendimiento se obtienen de los mismos sitios de celda repetidamente durante días.

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Cite this Article

Zlochiver, V., Kroboth, S. L., Beal, C. R., Cook, J. A., Joshi-Mukherjee, R. Human iPSC-Derived Cardiomyocyte Networks on Multiwell Micro-electrode Arrays for Recurrent Action Potential Recordings. J. Vis. Exp. (149), e59906, doi:10.3791/59906 (2019).

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