Journal
/
Genética
/
Pooled CRISPR-Based Genetic Screens in Mammalian Cells
JoVE Journal
Genética
É necessária uma assinatura da JoVE para visualizar este conteúdo.  Faça login ou comece sua avaliação gratuita.
JoVE Journal Genética
Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

20,786 Views

•

00:09 min

•

September 04, 2019

DOI:

10.3791/59780-v

DOI:
10.3791/59780-v

•

00:09 min
September 04, 2019

•

13 Views
, , , ,
1Donnelly Centre,University of Toronto, 2Department of Molecular Genetics,University of Toronto, 3Institute for Biomaterials and Biomedical Engineering,University of Toronto

Summary

Automatically generated

CRISPR-Cas9 technology provides an efficient method to precisely edit the mammalian genome in any cell type and represents a novel means to perform genome-wide genetic screens. A detailed protocol discussing the steps required for the successful performance of pooled genome-wide CRISPR-Cas9 screens is provided here.

Explore More Videos

CRISPRGenetic ScreensMammalian CellsDrop-out ScreensGuide LibrariesEssential GenesCancerDrug MechanismsCell LinesLentivirusAntibiotic SelectionPlasmid TransformationElectrocompetent CellsLBCarbenicillinPlasmid Purification293T CellsTransfection

Read Article

Cite this Article

Chan, K., Tong, A. H. Y., Brown, K. R., Mero, P., Moffat, J. Pooled CRISPR-Based Genetic Screens in Mammalian Cells. J. Vis. Exp. (151), e59780, doi:10.3791/59780 (2019).

Download .ris file

Copy

Share Video

.

Copy