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Pooled CRISPR-Based Genetic Screens in Mammalian Cells
JoVE 杂志
遗传学
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JoVE 杂志 遗传学
Pooled CRISPR-Based Genetic Screens in Mammalian Cells

Pooled CRISPR-Based Genetic Screens in Mammalian Cells

20,680 Views

00:09 min

September 04, 2019

DOI:

00:09 min
September 04, 2019

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Summary

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CRISPR-Cas9 technology provides an efficient method to precisely edit the mammalian genome in any cell type and represents a novel means to perform genome-wide genetic screens. A detailed protocol discussing the steps required for the successful performance of pooled genome-wide CRISPR-Cas9 screens is provided here.

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