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量化龙脑中的亚细胞泛基蛋白-蛋白酶体活性
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Neurociência
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JoVE Journal Neurociência
Quantifying Subcellular Ubiquitin-proteasome Activity in the Rodent Brain
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量化龙脑中的亚细胞泛基蛋白-蛋白酶体活性

DOI:
10.3791/59695-v

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09:25 min

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May 21, 2019

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, ,
1Department of Animal and Poultry Sciences,Virginia Polytechnic Institute and State University, 2School of Neuroscience,Virginia Polytechnic Institute and State University

Capítulos

  • 00:04Título
  • 00:44Nuclear and Cytoplasmic Extraction
  • 02:31Synaptic Fraction Collection
  • 03:45Proteasome Activity Assay
  • 06:36Quantification of Linkage-specific Protein Ubiquitination
  • 07:34Results: Differential Subcellular Changes in Proteasome Activity and Protein Polyubiquitination in the Rat Amygdala Following Learning
  • 09:13Conclusion

Summary

Tadução automática

Tadução automática

该协议旨在有效地量化啮齿动物大脑不同细胞舱中的泛泛蛋白-蛋白酶体系统(UPS)活性。用户能够检查UPS在同一动物中核、细胞质和突触分数的功能,从而减少执行这些复杂分析所需的时间和动物数量。

Tags

Subcellular FractionationUbiquitin-proteasome SystemProtein UbiquitinationProteasome ActivityRodent BrainCytoplasmic FractionNuclear FractionSynaptic FractionCentrifugation

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