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小鼠肝外胆管有机体的产生

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Biologia do Desenvolvimento

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JoVE Journal Biologia do Desenvolvimento

Generation of Organoids from Mouse Extrahepatic Bile Ducts

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小鼠肝外胆管有机体的产生

DOI:

10.3791/59544-v

•

09:13 min

•

April 23, 2019

•

Junya Shiota, Nureen H. Mohamad Zaki, Juanita L. Merchant², Linda C. Samuelson², Nataliya Razumilava

¹Departments of Internal Medicine,The University of Michigan, ²Molecular & Integrative Physiology,The University of Michigan

Capítulos

00:04Título
00:58Extrahepatic Bile Duct (EHBD) Isolation
02:36Biliary Organoid Culture
03:48EHBD Organoid Passage and Storage
05:01EHBD Organoid Processing for Paraffin Embedding
07:07Results: Representative EHBD Characterization and Analysis
08:24Conclusion

Summary

Tadução automática

该方案描述了小鼠肝外胆管三维器官系统的产生。这些胆道有机物质可以维持在培养中, 以研究胆管细胞生物学。胆道有机体表达的标志物的祖先和胆道细胞, 是由偏振上皮细胞。

Tags

Keywords: Organoids Extrahepatic Bile Ducts Murine Cholangiopathies Preclinical Models Cholangiocyte Cell-to-cell Interactions Bile Duct Isolation Dissociation Basement Matrix Cell Culture

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